Iodination of Antibodies with Immobilized Iodogen

Microfluidic one-step synthesis of alginate microspheres immobilized with antibodies.

Micrometre- and submicrometre-size functionalized beads are frequently used to capture targets of interest from a biological sample for biological characterizations and disease diagnosis. The main challenge of the microbead-based assay is in the immobilization of probe molecules onto the microbead surfaces. In this paper, we report a versatile droplet microfluidics method to fabricate alginate ...

Binding kinetics of immobilized antibodies in a flow immunosensor

This study investigates the binding kinetics of immobilized antibody in a solid-phase displacement immunosensor conducted in flow under nonequilibrium conditions. The experimental system studied employs a monoclonal antibody, specific for cocaine and its metabolite, benzoylecgonine, immobilized onto agarose beads. After saturation of antibody binding sites with the ftuorophore-labeled antigen, ...

Iodination of Tyrosyls in Thyroglobulin

Iodination of nucleic acids in organic solvents with iodine monochloride.

Iodine monochloride, labeled with 1z51, iodinated ribonucleic acid and deoxyribonucleic acid dissolved as hexadecyltrimethylammonium salts in anhydrous dimethylformamide or N-ethyl acetamide. In the case of RNA (polyuridylate, polycytidylate, and soluble RNA) 5-iodouridine S/-phosphate, 8-iodoguanosine 5’-phosphate, and 5-iodocytidine 5’-phosphate are found. After 24 hours of reaction, 32% of U...

[Enzymatic iodination of thyroglobulins obtained from patients with thyroid disease].

Iodination of the isolated thyroglobulin (Tg) by peroxidase was compared with various Tg preparations obtained from patients with thyroid diseases. For the purpose, the iodination process was observed in the incubation medium containing Tg, iodide, H2O2-generating system, and thyroid peroxidase (TPO) or lactoperoxidase (LPO). During the incubation, iodination of Tg preparations increased gradua...